H014
A SARS-CoV-2-neutralizing antibody.
General information
H014 is a humanized murine monoclonal antibody targeting SARS-CoV-2 Spike protein's RBD in the open conformation developed using a phage display technique (Lv et al., 2020). H014 binds to Spike protein trimers of B.1.1.7 and B.1.351 without a reduction in affinity. For a standard version of Spike protein, the antibody displayed cooperative binding with antibodies HB27, P17, and FC05 (Sun et al., 2021; Wang et al., 2020; Yao et al., 2020).
Supporting references
| Link | Tested on | Impact factor | Notes | Publication date |
|---|---|---|---|---|
|
Structural basis for neutralization of SARS-CoV-2 and SARS-CoV by a potent therapeutic antibody
Spike protein Animal model In vitro Antibody |
in vitro binding assay; Vero cells; hACE2-humanized mice; SARS-CoV-2 strain BetaCoV/Beijing/AMMS01/2020 | 41.85 | The humanized monoclonal antibody neutralizes SARS-CoV-2 live- and pseudovirus at nanomolar concentrations. It binds RBD of Spike protein in the open conformation and thereby inhibits the virus' attachment to the host cell. The antibody is effective against developement of lung pathology in a hACE2 mouse model of SARS-CoV-2 infection. |
Sep/18/2020 |
|
Structure-based development of human antibody cocktails against SARS-CoV-2
Spike protein Biophysical assay Animal model In vitro Antibody |
in vitro biophysical assay; 293T cells (cell-to-cell fusion assay); Vero cells; Huh7 cells; hACE2-mice; BALB/c mice | 20.51 | The antibody binds to the SARS-CoV-2 spike protein synergistically with FC05. |
Dec/01/2020 |
|
Structure-based development of three- and four-antibody cocktails against SARS-CoV-2 via multiple mechanisms
Spike protein Cryo-EM Biophysical assay In vitro Antibody |
in vitro biophysical assay; cryo-EM | 20.51 | H014 bound to Spike protein trimers of B.1.1.7 and B.1.351 without a reduction in affinity. For a standard version of Spike protein, the antibody displayed cooperative binding with antibodies HB27, P17, and FC05. |
Mar/29/2021 |
