CTC-445.2
A SARS-CoV-2 spike protein RBD binder.
General information
CTC-445.2 is a monomeric polypeptide, derived from modified human ACE2 receptor, with higher affinity for the SARS-CoV-2 spike protein RBD, intended to be used as a decoy for the virus. The decoy was designed to be inert to the host, selected using yeast display technique for its affinity to RBD and selected to be more stable using directed evolution (Linsky et al., 2020).
Supporting references
| Link | Tested on | Impact factor | Notes | Publication date |
|---|---|---|---|---|
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Denovo design of potent and resilient hACE2 decoys to neutralize SARS-CoV-2
Spike protein ACE2 Protein factor Animal model In vitro |
hACE2 HEK 293T cells; Calu-3 cells; Balb/c mice; Syrian hamster model for SARS-CoV-2 infection | 41.85 | The monomeric SARS-CoV-2 spike protein RBD decoy is thermodynamically hyperstable (Tm ca. 93°C), manifests low nanomolar affinity for the RBD (KD ca. 21 nM) and can efficiently compete with the human ACE2. The decoy is resilient to RBD mutational escape. |
Nov/05/2020 |
